Portugal's record scorer was shown a straight red card four minutes from time for elbowing an opponent when his side were 2-0 down before they scored a late consolation.
The 39-year-old scored back-to-back hattricks in three days.
Al Nassr striker Cristiano Ronaldo has once again silenced his critics after grabbing a hat-trick. The 39-year-old scored three second half goals against Al Tai to bring up 33 goals in all competitions this season for the Saudi outfit.
Cristiano Ronaldo, who had just recently become the first footballer to achieve 800 career wins, was included in the Portugal squad for the upcoming friendly matches against Sweden and Slovenia.
The 39-year-old Portuguese was the only Al Nassr player to score in the shootout as his Saudi side went out 3-1 on penalties, the tie having ended 4-4 on aggregate.
Nassr, who won last week's first leg 1-0 thanks to a Ronaldo goal nine minutes from time, were the better side at Al Awwal Park and took the lead on 17 minutes through Portuguese winger Otavio.
Cristiano Ronaldo scored his 21st goal of the season and his fourth in the Asian Champions League on Wednesday as his moment of magic proved decisive in Al Nassr beating Al Fayha 1-0 in an all-Saudi last 16 tie.
Al Nassr crushed Inter Miami 6-0 as injury ruled Cristiano Ronaldo out of a much-hyped showdown with Lionel Messi, who was limited to a late substitute appearance in Thursday's friendly in Riyadh.
Luis Castro, Cristiano Ronaldo’s coach at Al Nassr, thanked the fans from Bangladesh for their “dedication and loyalty” towards the Saudi Pro League outfit.
About to turn 39 in two weeks’ time, Cristiano Ronaldo said that he was proud to be able to beat the likes of Erling Haaland in terms of goals scored in the previous year.
Chinese football fans raced on Wednesday to snap up tickets to see Cristiano Ronaldo play a rare match in their home country, with multiple online platforms selling out within a few hours.
He will turn 39 next February. Approaching an age even long before which athletes are removed from their peak, Cristiano Ronaldo does not seem to be stopping any time soon.
Messi and Ronaldo, who have faced each other more than 30 times in their careers, have won 13 Ballon d'Or awards between them, with the Argentine leading that race eight to five.
Nassr ended the group stage unbeaten, with four victories and two draws.
Cristiano Ronaldo has still got it.
Inter Miami was named the guest international side and will join Al-Hilal and Al Nassr in next February's Riyadh Season Cup.
Former Liverpool striker Sadio Mane and Anderson Talisca also scored for the Saudi side late Tuesday as they made it three wins from three in the group stages of the premier Asian club competition.
Senin Sebai scored on the break to put the club from Dushanbe in front with a minute left in the first half and Istiklol held out under relentless pressure until Ronaldo levelled with a clever clipped finish in the 66th minute.
Cristiano Ronaldo made the most of both awful defending and a plume of smoke to set Al-Nasr on the way to a 4-3 victory against Al-Ahli in the Saudi Pro League.