Messi returned to the starting line-up after skipping Saturday's 5-2 defeat at Atlanta due to muscle fatigue but he had to leave the field in the 38th minute, looking uncomfortable
It isn't yet known if either Messi or Alba will be available Wednesday, though both trained in full on Tuesday. With two regular-season matches slated before Miami plays in the U.S. Open Cup final on Sept. 27, Messi and/or Alba could be given further time to recuperate
Messi and his former Barcelona team-mate Jordi Alba both sat out the game with Martino saying it would have been "very reckless" for the Argentine to play in Atlanta
Messi, who has had a hectic schedule with his club Inter Miami, watched his teammates from the bench but was not listed as a substitute, having complained of tiredness after scoring the winner in Thursday's 1-0 victory over Ecuador.
Messi was one of eight Inter Miami players away on international duty, but Gerardo "Tata" Martino's team showed their strength in depth as they extended their unbeaten run in all competitions to 12 games.
"If you love Cristiano Ronaldo, you don’t have to hate (Lionel) Messi. We have both changed the history of world football,” Ronaldo said during an interview during with Record, a Portuguese newspaper.
Messi, who recently left Paris Saint-Germain for Inter Miami, is in with a chance of succeeding Karim Benzema and winning the Ballon d'Or for the eighth time in his remarkable career after leading Argentina to World Cup glory in Qatar last year
Lionel Messi and Inter Miami face the MLS champions as their bid to make the playoffs takes them to Los Angeles on Sunday.
Coach Lionel Scaloni's 32-man squad also features stalwarts including Angel Di Maria, who returned to Benfica this year, and defender Nicolas Otamendi.
It was Messi's 11th goal in all competitions since joining Miami (6-14-3, 21 points) July 15 and sealed the club's first win since May 13. Before Messi sealed the win, Miami had been 0-8-3 in its previous 11 MLS matches
Miami trailed 2-0 with 22 minutes left before Messi whipped in a free-kick from the left which was headed home by Leonardo Campana
"Very happy to get the first title in this club's history. Everyone's hard work and commitment made it possible. Hopefully this is just the beginning," Messi posted on Instagram
Messi overtook former Barcelona teammate Dani Alves who had won 43 trophies in his career.
Messi had put Miami ahead in the 24th minute with a wonder strike into the top corner but Fafa Picault levelled for Nashville in the second half
The trio emerged as the leading vote-getters from a jury comprised of coaches of clubs in UEFA competitions, those in charge of European national teams and a select group of journalists
Inter Miami coach Gerardo "Tata" Martino played down injury concerns over Lionel Messi on Monday as the resurgent MLS club prepared for a Leagues Cup semi-final clash with Philadelphia.
Messi scored in his third consecutive match for Inter in a 3-1 victory over Orlando City last week, helping them move into the last 16 of the tournament.
Orlando City head coach Oscar Pareja says his team's defeat to Lionel Messi's Inter Miami on Wednesday was a "circus" and said that the Argentine should have been sent off.
Both finishes came from close range from the Argentine who made it three wins out of three at a team which was winless in 11 league games before his arrival.