Messi returned to the starting line-up after skipping Saturday's 5-2 defeat at Atlanta due to muscle fatigue but he had to leave the field in the 38th minute, looking uncomfortable
It isn't yet known if either Messi or Alba will be available Wednesday, though both trained in full on Tuesday. With two regular-season matches slated before Miami plays in the U.S. Open Cup final on Sept. 27, Messi and/or Alba could be given further time to recuperate
Messi and his former Barcelona team-mate Jordi Alba both sat out the game with Martino saying it would have been "very reckless" for the Argentine to play in Atlanta
Messi, who has had a hectic schedule with his club Inter Miami, watched his teammates from the bench but was not listed as a substitute, having complained of tiredness after scoring the winner in Thursday's 1-0 victory over Ecuador.
Messi was one of eight Inter Miami players away on international duty, but Gerardo "Tata" Martino's team showed their strength in depth as they extended their unbeaten run in all competitions to 12 games.
"If you love Cristiano Ronaldo, you don’t have to hate (Lionel) Messi. We have both changed the history of world football,” Ronaldo said during an interview during with Record, a Portuguese newspaper.
Messi, who recently left Paris Saint-Germain for Inter Miami, is in with a chance of succeeding Karim Benzema and winning the Ballon d'Or for the eighth time in his remarkable career after leading Argentina to World Cup glory in Qatar last year
Lionel Messi and Inter Miami face the MLS champions as their bid to make the playoffs takes them to Los Angeles on Sunday.
Coach Lionel Scaloni's 32-man squad also features stalwarts including Angel Di Maria, who returned to Benfica this year, and defender Nicolas Otamendi.