Centre-back Pepe missed Portugal's training on Monday for the Euro 2016 semi-final against Wales because of muscle pain, the Portuguese Football Federation (FPF) announced.
Pulling the Real Madrid player out of training was just a precautionary measure ahead of Wednesday's match in Lyon, the federation said.
The 33-year-old was the only player missing from the session for Fernando Santos' men. Midfielder Andre Gomes and left-back Raphael Guerreiro returned from knocks over the weekend.
"I was pleased with the way all my players played, but I have to say that Pepe had a huge match," said Santos, after Pepe's performance in the quarter-final win over Poland on penalties.
Gomes insisted that he had fully recovered from a muscle problem that kept him out of the Poland game and was ready for the Wales clash.