England defender Phil Jagielka has been ruled out of Tuesday's Euro 2012 qualifier against Montenegro after suffering a hamstring injury in training on Friday.
Jagielka, who has been replaced by Bolton centre-back Gary Cahill, had been in contention to start the match at Wembley after impressive displays in England's last two matches against Bulgaria and Switzerland.
But the Everton star missed a training game at Arsenal's London Colney complex on Friday after experiencing tightness in his hamstring during the previous session.
A scan confirmed Jagielka would not be fit to face Montenegro, with England coach Fabio Capello now likely to restore Rio Ferdinand and John Terry to the centre of his defence after the pair missed the team's last two qualifiers.
Meanwhile, Manchester City midfielder Gareth Barry joined up with the England squad on Friday after being given additional time at home due to personal reasons.