Djokovic said that his subdued performance on a court where he has won six titles might have been due to the blow suffered while greeting fans on Friday night.
Djokovic's bid for a record-extending 41st Masters 1000 title was ended in just over an hour by Chilean Tabilo, who is ranked 32nd in the world but was hugely impressive and claimed his first win over a top-10 opponent.
Defeat to seventh seed Hurkacz casts doubt over whether clay-court icon Nadal will play at the upcoming French Open, where he has won a record 14 titles.
Novak Djokovic was left in agony after being struck with a hard plastic water bottle as he left the court following his 6-3, 6-1 win over France's Corentin Moutet at the Rome Open which advanced him to the third round on Friday.
"I have Roland Garros in just two weeks and a half... I need to prove myself if I am able to push my body to the limit, I need to push to feel myself ready for what's coming," Nadal told reporters.
World number one Novak Djokovic said he believes he will be in top form for the French Open and hopes to prove that at the Italian Open which started on Wednesday.
The 22-time Grand Slam singles champion has recently returned to the circuit after a long absence due to injury.
Rublev, who takes the Madrid crown from double champion Carlos Alcaraz whom he beat in the quarter-finals, said he had played despite feeling ill at times this week and hailed his doctors for helping him through.
Rafa Nadal continued his return to form as he defeated Alex de Minaur 7-6(6), 6-3 in the second round of the Madrid Open on Saturday.
Nadal, a 22-time Grand Slam champion, will be 38 in June and has made just a handful of appearances since January 2023 due mostly to a hip injury.
Defending Madrid Open champions Carlos Alcaraz and Aryna Sabalenka both triumphed in their opening clashes in the Spanish capital on Friday to reach the third round.
The win was Nadal's record-extending 57th victory in Madrid.
The three-time Wimbledon winner was sentenced to two-and-a-half years in prison for concealing assets in April 2022 but was released in December of the same year and subsequently deported to Germany.
Nadal was also 16 when he played the event in the Spanish capital for the first time.
The 37-year-old, a 22-time Grand Slam champion, said that he is far from being in top form and that he is only playing against American Darwin Blanch in the first round of the Madrid Open on Thursday as a personal sacrifice.
The Italian world number two can come within touching distance of the top-ranked Djokovic in the standings should he triumph in Madrid this fortnight and he arrives in the Spanish capital brimming with confidence and carrying an impressive 25-2 win-loss record for the season.
Djokovic has now pulled out of the Madrid Open three times in four years.
World number six Casper Ruud defeated Stefanos Tsitsipas 7-5, 6-3 to win the Barcelona Open on Sunday.